Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rheumatic Heart Disease I: Introduction01:23

Rheumatic Heart Disease I: Introduction

Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
Pericarditis I: Introduction01:22

Pericarditis I: Introduction

Pericarditis is defined as the inflammation of the pericardium, the thin, sac-like membrane surrounding the heart. This condition can cause significant chest pain and other symptoms, often necessitating medical intervention. The pericardium has two layers: the inner visceral layer and the outer parietal layer, separated by a small amount of fluid that reduces friction during heartbeats.Types of PericarditisPericarditis can be classified into several types based on the duration and nature of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.

Biomolecules·2026
Same author

MYH9 mutations differentially stabilize non-muscle myosin II filaments and induce distinct cellular aggregation phenotypes.

Cellular and molecular life sciences : CMLS·2026
Same author

Deep learning-based plaque characterization in hybrid IVUS-OCT images is superior to single-modality deep learning analysis and human experts: head-to-head comparison against histology.

Cardiovascular research·2026
Same author

Pulmonary valve repair at the time of the Ross procedure: A safe and durable strategy to address postimplantation aortic regurgitation.

The Journal of thoracic and cardiovascular surgery·2025
Same author

Molecular Control of Non-Muscle Myosin II-A Aggregation and Intracellular Dynamics by motor- or tail-specific <i>MYH9</i> Mutations.

bioRxiv : the preprint server for biology·2025
Same author

Learning curve of the Ross procedure after more than 650 interventions: a single-centre, retrospective analysis†.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2025

Related Experiment Video

Updated: Jun 21, 2026

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
13:45

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue

Published on: November 11, 2022

Second harmonic generation microscopy to investigate collagen configuration: a pericarditis case study.

Jonathan Bélisle1, Tiffany Zigras, Santiago Costantino

  • 1Department of Physics, McGill University, Montreal, Canada.

Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology
|July 28, 2009
PubMed
Summary

Second-harmonic-generation (SHG) imaging visualizes collagen in pericardial tissue without staining. This technique effectively distinguishes mature from newly accumulated collagen in constrictive pericarditis.

More Related Videos

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

Published on: January 6, 2018

Related Experiment Videos

Last Updated: Jun 21, 2026

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
13:45

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue

Published on: November 11, 2022

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

Published on: January 6, 2018

Area of Science:

  • Biomedical Optics
  • Histopathology
  • Cardiovascular Research

Background:

  • Constrictive pericarditis involves collagen remodeling in the pericardium.
  • Accurate assessment of collagen architecture is crucial for understanding pericardial diseases.
  • Traditional histology requires staining and can be time-consuming.

Observation:

  • Second-harmonic-generation (SHG) microscopy was employed to image collagen fibers in human pericardial tissue.
  • SHG imaging provided label-free visualization of collagenous structures.
  • Images obtained via SHG were directly compared with conventional stained histology slides.

Findings:

  • SHG imaging successfully visualized collagen fiber organization in both healthy and diseased pericardium.
  • The collagen structure and morphology observed in SHG images were consistent with those in stained histology.
  • SHG microscopy clearly differentiated mature parietal pericardium collagen from newly accumulated collagen associated with pericarditis.

Implications:

  • SHG imaging offers a rapid, label-free method for assessing pericardial collagen.
  • This technique can provide valuable insights into the architectural changes of collagen in constrictive pericarditis.
  • SHG microscopy holds potential for improved diagnostic and research applications in cardiovascular pathology.