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Related Experiment Video

Updated: Jun 21, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Differential expression and function of ABCG1 and ABCG4 during development and aging.

Dragana D Bojanic¹, Paul T Tarr, Greg D Gale

¹Department of Biological Chemistry at UCLA Los Angeles, CA 90095, USA.

Journal of Lipid Research

|July 28, 2009

Summary

ATP binding cassette (ABC) transporters ABCG1 and ABCG4 exhibit distinct developmental expression patterns. Their loss in the central nervous system (CNS) alters cholesterol metabolism and impacts fear memory in mice.

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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

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Published on: June 3, 2016

Area of Science:

Molecular Biology
Neuroscience
Developmental Biology

Background:

ABCG1 and ABCG4 are homologous ATP binding cassette (ABC) transporters crucial for cellular cholesterol homeostasis.
In adult mice, ABCG1 has broad tissue expression, while ABCG4 is restricted to the central nervous system (CNS).

Purpose of the Study:

To investigate the differential developmental expression patterns of ABCG1 and ABCG4.
To determine the functional consequences of ABCG1 and ABCG4 loss in the CNS on cholesterol metabolism and behavior.

Main Methods:

Analysis of beta-galactosidase-stained tissue sections from Abcg1(-/-)LacZ and Abcg4(-/-)LacZ knockin mice.
Assessment of oxysterol accumulation, gene expression changes (liver X receptor, sterol-regulatory element binding protein-2), and behavioral tests (associative fear memory).

Main Results:

ABCG4 shows transient developmental expression in hematopoietic cells and enterocytes.
ABCG1 is expressed in embryonic and adult liver macrophages and endothelial cells.
Both transporters are expressed in the embryonic eye and developing CNS by E12.5.
Loss of ABCG1/ABCG4 leads to retinal/brain oxysterol accumulation, altered target gene expression, and a stress response.
Abcg4(-/-) mice exhibit deficits in associative fear memory.

Conclusions:

ABCG1 and ABCG4 display distinct developmental expression profiles.
Loss of these transporters in the CNS disrupts cholesterol metabolism and impacts cognitive functions, specifically fear memory.