Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amuc_1100 alleviates HFD-induced hepatic lipid accumulation via gut microbiota in zebrafish: insights from the role of intestinal 14-3-3β/α-A.

Journal of animal science and biotechnology·2026
Same author

Gel-Based NMR Method for Observing Submicrosecond Protein Dynamics at Atomic Resolution.

The journal of physical chemistry letters·2026
Same author

DEEP Phaser: A Deep Learning Tandem Vision Transformer for Fully Automated NMR Phase Correction.

The journal of physical chemistry letters·2026
Same author

Tolerance and response of afatinib combined with tegafur in patients with advanced biliary tract cancer after failure of gemcitabine-based systemic therapy.

Discover oncology·2026
Same author

The scientific legacy of Martin Karplus from the perspective of his collaborators.

Biophysical journal·2026
Same author

Fault Diagnosis Method for Reciprocating Compressors Based on Spatio-Temporal Feature Fusion.

Sensors (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jun 21, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Web server suite for complex mixture analysis by covariance NMR.

Fengli Zhang1, Steven L Robinette, Lei Bruschweiler-Li

  • 1National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, USA.

Magnetic Resonance in Chemistry : MRC
|July 28, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces COLMAR, a web server suite for analyzing complex biological mixtures using nuclear magnetic resonance (NMR) spectroscopy. COLMAR aids in identifying and quantifying metabolites, advancing systems biology and metabolomics research.

More Related Videos

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Related Experiment Videos

Last Updated: Jun 21, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Computational Biology

Background:

  • Systems biology and metabolomics require accurate identification and quantification of metabolites in biological samples.
  • Nuclear magnetic resonance (NMR) spectroscopy offers rich molecular information for metabolite analysis.
  • Existing methods for complex mixture analysis can be inefficient or lack automation.

Purpose of the Study:

  • To present COLMAR, a suite of public web servers designed for analyzing complex mixtures using NMR spectroscopy.
  • To provide reliable, efficient, and automatable tools for metabolite identification and quantification.
  • To demonstrate the utility of COLMAR for advancing systems biology and metabolomics.

Main Methods:

  • Development of the COLMAR web portal, comprising three integrated servers: COLMAR covariance, COLMAR DemixC, and COLMAR query.
  • COLMAR covariance calculates covariance NMR spectra from input datasets.
  • COLMAR DemixC decomposes 2D spectra into 1D cross sections for individual components, which are then screened against an NMR spectral database by COLMAR query for compound identification.

Main Results:

  • The COLMAR web server suite effectively facilitates the analysis of complex mixtures by NMR.
  • The integrated servers enable the decomposition of complex spectra and the identification of individual compounds.
  • Demonstrated utility of the COLMAR suite through practical examples of complex mixture analysis.

Conclusions:

  • The COLMAR web server suite provides a powerful and accessible platform for NMR-based metabolomics.
  • This tool enhances the ability to identify and quantify metabolites in complex biological samples.
  • COLMAR advances the field of systems biology by enabling more comprehensive molecular analysis.