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Published on: October 14, 2011
Tobacco streak virus isolated from lettuce
1Department of Plant Protection, Collage of Agriculture, University of Tehran, Karaj, Islamic Republic of Iran.
Pakistan Journal of Biological Sciences : PJBS
|July 29, 2009
Summary
Tobacco streak virus (TSV) was identified in lettuce fields in Tehran Province, causing mosaic and leaf distortion. This study confirmed TSV presence and characterized its effects on various plant species.
Area of Science:
- Plant Pathology
- Virology
- Agricultural Science
Background:
- Tobacco streak virus (TSV) is a widespread ilarvirus causing significant crop losses globally.
- Lettuce (Lactuca sativa) is susceptible to various viral diseases, impacting yield and quality.
Purpose of the Study:
- To identify and characterize Tobacco streak virus (TSV) isolates from infected lettuce in Tehran Province, Iran.
- To determine the host range and symptoms induced by TSV isolates on different plant families.
Main Methods:
- Collection and analysis of 300 lettuce samples from Tehran Province.
- Detection of TSV using Double Antibody Sandwich-ELISA (DAS-ELISA) with polyclonal antiserum.
- Inoculation of five TSV isolates onto 29 plant species from various families (Cucurbitaceae, Amaranthaceae, Solanacea, Compositae, Leguminosae, Chenopodiacea).
- Symptom observation and documentation.
- Confirmation of TSV presence using Reverse Transcription Polymerase Chain Reaction (RT-PCR) with specific primers.
Main Results:
- TSV infection in lettuce presented symptoms including mosaic, vein clearing, vein necrosis, yellowing, and leaf distortion.
- TSV isolates induced varied reactions in inoculated plants: necrotic local lesions in Chenopodium quinoa and Phaseolus vulgaris cv. Red Kidney, chlorotic local lesions in Gomphrena globosa, systemic symptoms in Datura stramonium, and necrotic/chlorotic local lesions in Nicotiana tabacum.
- Nicotiana clevelandii developed leaf distortion and vein necrosis, while Lactuca sativa exhibited leaf distortion and mosaic.
- RT-PCR successfully amplified a specific ~710 bp fragment, confirming TSV presence.
Conclusions:
- The study confirmed the presence of Tobacco streak virus (TSV) in lettuce crops in Tehran Province, Iran.
- TSV isolates demonstrated a broad host range, affecting species across multiple plant families.
- The findings highlight the potential threat of TSV to agricultural production in the region and underscore the need for effective management strategies.
