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Gemcitabine impacts histological structure of mice testis and embryonic organs
1Department of Zoology, Girl's College of Science, University of King Abdul Aziz, Jeddah, Saudi Arabia.
Abstract:
The present study aimed to investigate the toxic impact of gemcitabine on the histological structure of white mice testis and the histological structure of some embryonic organs. The mature male mice were treated with 130 mg kg(-1) of gemcitabine intraperitoneally. These treated males were placed after one week with mature normal females for fertilization. Females were dissected after the 14th day of pregnancy to investigate embryos. The histological investigation of testis showed an interruption in spermatogenesis process as evident by distorted spermatocytes, spermatozoa and a reduction in their number. Histological examination of embryonic organs, including liver, kidney, small intestine, spleen, zatrek gland and testis, after two weeks of pregnancy revealed impaired structures. The drug also reduced fertility and survival of embryos. In conclusion, present study suggests that cautions should be taken when gemcitabine is used as an anticancer drug.
Insights
Gemcitabine chemotherapy significantly damages white mice testis histology, impairing sperm production and embryo development. This anticancer drug reduces fertility and embryo survival, necessitating cautious use.
Area of Science:
- Toxicology
- Histopathology
- Reproductive Biology
Background:
- Gemcitabine is a widely used chemotherapy agent.
- Potential reproductive toxicity of gemcitabine requires thorough investigation.
Purpose of the Study:
- To evaluate the toxic effects of gemcitabine on male mouse reproductive organs.
- To assess the impact of gemcitabine exposure on embryonic development and organogenesis.
Main Methods:
- Mature male mice received intraperitoneal injections of gemcitabine (130 mg/kg).
- Treated males were mated with normal females; pregnant females were dissected on day 14.
- Histological examination of testes and embryonic organs (liver, kidney, intestine, spleen, thymus, testis) was performed.
Main Results:
- Gemcitabine induced significant testicular damage, including disrupted spermatogenesis, reduced sperm count, and abnormal sperm morphology.
- Histological analysis revealed structural impairments in embryonic organs such as the liver, kidney, and intestines.
- Fertility rates and embryo survival were markedly reduced in gemcitabine-exposed groups.
Conclusions:
- Gemcitabine exhibits considerable toxicity to male reproductive tissues and developing embryos.
- The findings highlight the need for careful consideration of gemcitabine's reproductive risks in clinical settings.
