Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 1000<sup>+</sup> mouse project for large-scale spatiotemporal parametrization and modeling of preclinical cancer immunotherapies.

eLife·2026
Same author

The 1000<sup>+</sup> mouse project: large-scale spatiotemporal parametrization and modeling of preclinical cancer immunotherapies.

bioRxiv : the preprint server for biology·2025
Same author

Correction: Novel humanized CD19-CAR-T (Now talicabtagene autoleucel, Tali-cel™) cells in relapsed/ refractory pediatric B-acute lymphoblastic leukemia- an open-label single-arm phase-I/Ib study.

Blood cancer journal·2025
Same author

Novel humanized CD19-CAR-T (Now talicabtagene autoleucel, Tali-cel™) cells in relapsed/ refractory pediatric B-acute lymphoblastic leukemia- an open-label single-arm phase-I/Ib study.

Blood cancer journal·2025
Same author

Engineering TCR-controlled fuzzy logic into CAR T cells enhances therapeutic specificity.

Cell·2025
Same author

Talicabtagene autoleucel for relapsed or refractory B-cell malignancies: results from an open-label, multicentre, phase 1/2 study.

The Lancet. Haematology·2025

Related Experiment Video

Updated: Jun 21, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
08:10

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Peripheral blood based C-PCR assay for diagnosing extra-pulmonary tuberculosis.

Rajiv Khosla1, Alka Dwivedi, B C Sarin

  • 1Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143 005, India.

Indian Journal of Experimental Biology
|July 29, 2009
PubMed
Summary

Diagnosing extra pulmonary tuberculosis (EPTB) in India is challenging. Competitive PCR (C-PCR) in peripheral blood shows promise for detecting Mycobacterium tuberculosis, offering quantitative insights beyond traditional methods.

More Related Videos

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
09:54

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Related Experiment Videos

Last Updated: Jun 21, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
08:10

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
09:54

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Extra pulmonary tuberculosis (EPTB) accounts for 20% of tuberculosis cases in India.
  • Conventional diagnostic methods for EPTB have limitations in sensitivity and specificity.
  • Polymerase chain reaction (PCR) offers improved detection but lacks quantitative capabilities.

Purpose of the Study:

  • To evaluate peripheral blood as a diagnostic specimen for EPTB.
  • To assess the utility of a novel Competitive PCR (C-PCR) assay for quantifying Mycobacterium tuberculosis in blood.
  • To compare PCR-based methods with conventional mycobacterial culture for EPTB diagnosis.

Main Methods:

  • Analysis of peripheral blood samples from 38 EPTB patients and 89 non-tuberculous controls using MPB 64 gene-based PCR.
  • Evaluation of an in-house Competitive PCR (C-PCR) assay to determine mycobacterial load (genome equivalents/mL).
  • Comparison of diagnostic performance metrics (sensitivity, negative predictive value) against mycobacterial culture.

Main Results:

  • The MPB 64 gene-based PCR showed a sensitivity of 60.53% and NPV of 76.92%, outperforming mycobacterial culture (10.53% sensitivity, 72.36% NPV).
  • A significant proportion (43.82%) of non-tuberculous subjects tested positive with the standard PCR, limiting its clinical utility.
  • C-PCR successfully quantified mycobacterial load in peripheral blood, with distinct ranges observed for culture-positive EPTB, culture-negative EPTB, and controls.

Conclusions:

  • Peripheral blood is a viable specimen for EPTB diagnosis.
  • Competitive PCR (C-PCR) offers a quantitative advantage for diagnosing EPTB.
  • C-PCR provides valuable insights into mycobacterial load, aiding in EPTB diagnosis.