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Updated: Jun 21, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
Published on: December 17, 2013
[Molecular interaction between PrP protein and the signal protein 14-3-3 beta]
Guo-yong Mei1, Yuan Li, Gui-rong Wang
1Pathogenic Microorganisms Department, Medical School, Anhui University of Science and Technology, Huainan, 232001, China. 06mgy@163.com
Abstract:
The molecular interaction between PrP and 14-3-3 beta and the possible interactional domain between two proteins were studied by co-immunoprecipitation, pull down and FRET assays. The results showed that PrP protein could interact with 14-3-3 beta in vitro and in vivo. The domain which responded for the interaction was located at C-terminal of PrP (amino acid residues 106 to 126). This study of the interaction between PrP and 14-3-3 protein further provided the insight into the potential role of 14-3-3 in the biological function of PrP and the pathogenesis of prion disease.
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