Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

Area of Science:

• Veterinary Microbiology
• Biotechnology
• Analytical Chemistry

Background:

• Brucellosis remains a significant zoonotic disease globally.
• Accurate and rapid on-site detection methods for Brucella are crucial for disease control.
• Existing diagnostic techniques can be time-consuming or require specialized laboratory equipment.

Purpose of the Study:

• To develop and validate a rapid, quantitative, on-site assay for Brucella detection.
• To evaluate the performance of the up-converting phosphor technology-based lateral-flow (UPT-LF) assay.
• To assess the assay's applicability for various sample types, including pure cultures and spiked samples.

Main Methods:

• Development of a novel up-converting phosphor technology-based lateral-flow assay (UPT-LF assay).
• Quantitative detection of different Brucella species in pure cultures.
• Analysis of Brucella in spiked biological or environmental samples.

Main Results:

• The UPT-LF assay successfully detected and quantified Brucella species in pure cultures.
• Detection limit for pure cultures was 5 x 10^6 CFU/ml.
• Sensitivity for spiked samples ranged from 2.0 x 10^3 to 3.9 x 10^5 CFU/mg, demonstrating broad applicability.
• The assay exhibited high specificity, reproducibility, and stability.

Conclusions:

• The developed UPT-LF assay is a rapid and quantitative tool for on-site Brucella detection.
• The assay's performance characteristics indicate its suitability for field deployment.
• This technology holds significant potential for improving Brucella surveillance and control strategies.