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Updated: Jun 21, 2026
Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses
Published on: October 21, 2016
A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format
Anders R Johnsen1, Trine K Hybholt, Ole S Jacobsen
1Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. arj@geus.dk
Abstract:
A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.
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