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Related Experiment Video

Updated: Jun 21, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

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Published on: March 14, 2021

Lysophosphatidic acid acyltransferase 3 regulates Golgi complex structure and function.

John A Schmidt¹, William J Brown

¹Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

The Journal of Cell Biology

|July 29, 2009

Summary

Lysophosphatidic acid-specific acyltransferase 3 (LPAAT3) regulates Golgi structure and protein trafficking by remodeling membrane phospholipids. This study reveals LPAAT3

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Last Updated: Jun 21, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

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Published on: March 14, 2021

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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Published on: March 18, 2011

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Golgi complex organization is crucial for cellular function.
Phospholipid remodeling enzymes are implicated in Golgi complex organization.

Purpose of the Study:

To identify and characterize novel enzymes involved in Golgi complex functional organization.
To elucidate the role of lysophosphatidic acid-specific acyltransferase 3 (LPAAT3) in regulating Golgi membrane dynamics and protein trafficking.

Main Methods:

Identification of integral membrane proteins.
In vivo and in vitro assays for Golgi membrane tubule formation.
Analysis of protein trafficking using overexpression and siRNA knockdown of LPAAT3.
Assessment of Golgi morphology through microscopy.

Main Results:

LPAAT3 was identified as a lysophosphatidic acid-specific acyltransferase regulating Golgi membrane phospholipids.
Overexpression of LPAAT3 inhibited Golgi membrane tubule formation.
LPAAT3 modulated both anterograde and retrograde protein trafficking.
LPAAT3 knockdown resulted in Golgi fragmentation, indicating its role in maintaining Golgi structure.

Conclusions:

LPAAT3 directly regulates Golgi membrane tubule formation, protein trafficking, and overall organelle structure.
This study establishes a direct link between phospholipid remodeling and Golgi complex organization and function.