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Updated: Jun 21, 2026

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
In vitro 24-hour time-kill studies of vancomycin and linezolid in combination versus methicillin-resistant
Shveta Rani Singh1, Alfred E Bacon, David C Young
1Department of Medicine-Pediatrics, Christiana Care Health System, 4755 Ogletown-Stanton Road, Newark, Delaware 19718, USA. ssingh@christianacare.org
Abstract:
Many clinicians are trying unique strategies, including vancomycin and linezolid in combination, for treatment of patients who do not respond to conventional therapy against methicillin (meticillin)-resistant Staphylococcus aureus. In our study, which illustrated in vitro activity only, no synergistic activity was seen when the two agents were combined. Conversely, antagonistic activity occurred in three of five strains when linezolid was added to vancomycin. Our results indicate that vancomycin and linezolid in combination should be avoided.
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