Search research articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Published on: July 27, 2009

Batch immunostaining for large-scale protein detection in the whole monkey brain.

Shahin Zangenehpour¹, Mark W Burke, Avi Chaudhuri

¹Cognitive Neuroscience Unit, Montreal Neurological Institute. shahin.zangenehpour@mcgill.ca

Journal of Visualized Experiments : Jove

|July 29, 2009

Summary

This study presents a new method for immunohistochemistry (IHC) staining in large primate brains. This systematic approach ensures uniform protein detection across the entire brain for comprehensive analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evolution of the frontal aslant tract and implications for primate vocalization and human speech.

Nature communications·2026

Same author

Rapid sequential activation from A1 to V1 in congenitally blind and sighted subjects.

Brain research bulletin·2026

Same author

Pharmacokinetics of oral Δ9-tetrahydrocannabinol (THC) administration in vervet monkeys.

Journal of cannabis research·2026

Same author

Pathways of Cross-Modal Access to the Visual Cortex in Late Blindness.

Research square·2026

Same author

Impact of prenatal alcohol exposure in midlife: an assessment of the retina in the vervet monkey.

Experimental eye research·2025

Same author

Visual Prostheses in the Era of Artificial Intelligence Technology.

Eye and brain·2025

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Area of Science:

Neuroscience
Immunohistochemistry
Primate Brain Research

Background:

Immunohistochemistry (IHC) is crucial for detecting proteins in situ.
Analyzing protein expression in large, complex primate brains using IHC is challenging.
Rodent brain studies are simpler, but primate brain analysis requires specialized methods.

Purpose of the Study:

To develop a systematic approach for protein immunodetection in adult monkey brains.
To overcome challenges in applying IHC to large and convoluted primate brains.
To enable comprehensive analysis of protein expression patterns throughout the primate brain.

Main Methods:

Utilized a specialized tissue embedding and sectioning methodology (NeuroScience Associates).
Employed tools designed for batch-staining of free-floating brain sections.

More Related Videos

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Published on: April 5, 2012

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Published on: January 17, 2025

Related Experiment Videos

Last Updated: Jun 21, 2026

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Published on: July 27, 2009

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Published on: April 5, 2012

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Published on: January 17, 2025

Ensured uniform staining across a representative set of sections covering the entire brain.

Main Results:

Achieved uniform and consistent immunodetection of target proteins in adult monkey brains.
Developed a method applicable to large, convoluted brain structures.
Generated a comprehensive dataset of stained sections for further analysis.

Conclusions:

The presented systematic approach effectively addresses IHC challenges in primate brains.
This method allows for accurate measurement of protein levels and neuronal expression.
Enables detailed mapping of protein distribution across the entire primate brain.