Transcriptional analysis of the lysine-responsive and riboswitch-regulated lysC gene of Bacillus subtilis

About 2% of the Bacillus subtilis genes are subject to regulation by riboswitch-controlled mechanisms. One of them is the L-lysine-dependent lysC gene which is turned on when the L-lysine concentration within the cytoplasm is low. In the presence of a high L-lysine concentration, only a 0.27-kb transcript is synthesized representing the riboswitch due to transcription termination. When the L-lysine concentration is low, the full-length 1.6-kb transcript is produced due to transcription anti-termination. Here, we show for the first time that even under conditions of transcription anti-termination the truncated form of the RNA is still predominant. This 0.27-kb transcript is neither the result of enhanced stability nor does it result from processing of the full-length transcript. When the region coding for the transcription terminator was removed, the riboswitch RNA failed to be produced. These data were confirmed by analysis of a transcriptional fusion between the promoter-riboswitch region of lysC with and without a functional transcriptional terminator and the lacZ reporter gene. The putative function(s) of the riboswitch under conditions of low L-lysine concentration is discussed.