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[Karyological study of the HTR-5 cell line]
Tsitologiia I Genetika
|March 1, 1977
Summary
This study examined human fibroblasts altered by the SV-40 virus. The HTR-5 cell line primarily exhibits a pseudodiploid karyotype with distinctive marker chromosomes.
Area of Science:
- Cytogenetics
- Molecular Virology
- Cell Biology
Context:
- Investigating the genomic stability of human cell lines after viral transformation.
- Utilizing the Simian Vacuolating Virus 40 (SV-40) as a tool for cellular modification.
Purpose:
- To characterize the karyotypic profile of the SV-40 transformed human fibroblast cell line, HTR-5, at a later passage.
- To identify and describe any unique chromosomal abnormalities present in the HTR-5 cell line.
Summary:
- Karyologic analysis of the HTR-5 cell line, derived from SV-40 transformed human fibroblasts at the 15th subculture, revealed a predominantly pseudodiploid karyotype.
- The study identified specific marker chromosomes within the HTR-5 cell line, indicative of its transformed state and unique genetic alterations.
Impact:
- Provides a detailed cytogenetic characterization of a specific SV-40 transformed human fibroblast cell line (HTR-5).
- Contributes to the understanding of viral-induced chromosomal changes in human cells.
- Offers a characterized cell line for future research in cell cycle regulation, DNA repair, or oncogenesis.