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Updated: Jun 21, 2026

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
Sequence-specific 1H, 13C, and 15N backbone assignment of the GTPase rRheb in its GDP-bound form
Carsten Berghaus1, Melanie Schwarten, Rolf Heumann
1Biomolecular NMR, Faculty of Chemistry and Biochemistry, Ruhr-University of Bochum, Bochum 44780, Germany.
Abstract:
Rheb (Ras homologue enriched in brain) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of rRheb in its GDP-bound state. These assignments now provide a basis for the analysis of the interaction of rRheb with putative factors in order to elucidate the function of this GTPase and its role in the MAP kinase pathway of neuronal cells and in tuberous sclerosis.
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