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Updated: Jun 21, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Published on: March 31, 2012
Sequence-specific 1H, 13C, and 15N backbone assignment of the activated 21 kDa GTPase rRheb
Melanie Schwarten1, Carsten Berghaus, Rolf Heumann
1Biomolecular NMR, Ruhr-University of Bochum, Bochum 44780, Germany.
Abstract:
Ras homologue enriched in brain (Rheb) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of activated rRheb in complex with the non-hydrolisable GTP analogue GppNHp. These assignments now provide a basis for the analysis of rRheb's interaction with putative effectors in order to further elucidate the physiological function of this GTPase and its role in the regulation of neuronal cell volume as well as in tuberous sclerosis.
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