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Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Backbone assignment of human proliferating cell nuclear antigen.

Ricardo Sánchez1, Daniel Torres, Jesús Prieto

  • 1Spanish National Cancer Center, Madrid, Spain.

Biomolecular NMR Assignments
|July 29, 2009
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Summary

This study details the resonance assignments for human Proliferating Cell Nuclear Antigen (PCNA), a key protein in DNA replication and repair. Understanding PCNA

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Proliferating Cell Nuclear Antigen (PCNA) is crucial for DNA replication, repair, and cell-cycle regulation.
  • PCNA functions as a homotrimeric ring, playing a central role in cellular processes.

Purpose of the Study:

  • To assign the backbone and sidechain resonances of human PCNA.
  • To provide foundational data for further structural and functional studies of PCNA.

Main Methods:

  • Utilized Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Assigned backbone resonances: H(N), C(alpha), C(O), and N-15.
  • Assigned sidechain resonances: C(beta).

Main Results:

  • Complete assignment of backbone (1)H(N), (13)C(alpha), (13)CO, and (15)N resonances for human PCNA.
  • Assignment of sidechain (13)C(beta) resonances for the human PCNA homotrimer.
  • Characterized the approximately 90 kDa, 261-residue homotrimeric ring structure.

Conclusions:

  • The reported resonance assignments provide critical data for understanding human PCNA.
  • This work facilitates future investigations into PCNA's role in DNA metabolism and cell cycle control.