Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gastrointestinal adverse reactions and metabolism-nutrition disorders associated with hypomethylating agents: a pharmacovigilance study with exploratory mechanistic analysis.

Frontiers in nutrition·2026
Same author

Effect of Admission Diabetes History on Safety and Efficacy of Early Tirofiban Infusion after Intravenous Thrombolysis in Ischaemic Stroke: a post-hoc analysis of the ASSET-IT trial.

European stroke journal·2026
Same author

Impact of lymph node dissection on textbook outcomes in patients with intrahepatic cholangiocarcinoma: a multi-center analysis.

BMC gastroenterology·2026
Same author

A functional antiplatelet nanomotor for tri-modal ablation of acute arterial thrombus.

Cell reports. Medicine·2026
Same author

Ivonescimab plus gemcitabine and cisplatin as first-line therapy for advanced biliary tract cancer: a multicenter, open-label phase 2 trial.

Journal of hepatology·2026
Same author

Tailoring the Electron-Enriched Microenvironment of UiO-66 via Thiol Functionalization to Boost Non-Thermal Plasma CO<sub>2</sub> Conversion.

Inorganic chemistry·2026

Related Experiment Video

Updated: Jun 21, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

VIRS: A visual tool for identifying restriction sites in multiple DNA sequences.

Xiang Chen1, Cong Luo, Xiaoxia Ma

  • 1Dept. of Bioinformatics, Zhejiang University, Hangzhou, P.R. China.

Biotechnology Progress
|July 29, 2009
PubMed
Summary

VIRS is a web tool for predicting and visualizing restriction sites in DNA sequences. It offers custom analysis, virtual electrophoresis, and enzyme data, aiding molecular biology research.

More Related Videos

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Related Experiment Videos

Last Updated: Jun 21, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Area of Science:

  • Bioinformatics
  • Molecular Biology
  • Computational Biology

Background:

  • Restriction endonuclease analysis is crucial for DNA manipulation.
  • Existing tools often lack comprehensive visualization or simultaneous multi-sequence analysis.
  • Accurate prediction and visualization of restriction sites are essential for experimental design.

Purpose of the Study:

  • To develop an interactive web-based program, VIRS, for predicting and visualizing restriction endonuclease cut sites.
  • To enable simultaneous analysis of multiple DNA sequences.
  • To provide users with customizable analysis options and detailed enzyme information.

Main Methods:

  • Development of an interactive, web-based platform (VIRS).
  • Implementation of algorithms for restriction site prediction and visualization.
  • Integration of a comprehensive, internally maintained database of restriction endonucleases.
  • Inclusion of features for virtual electrophoresis of digested DNA fragments.

Main Results:

  • VIRS successfully predicts and visualizes restriction sites across multiple DNA sequences concurrently.
  • The tool offers customizable visual restriction maps and virtual gel electrophoresis outputs.
  • VIRS provides detailed properties of commercially available restriction enzymes from an updated database.

Conclusions:

  • VIRS enhances the analysis of restriction sites in DNA sequences through its visual and interactive capabilities.
  • The program serves as a valuable resource for molecular biologists by integrating prediction, visualization, and enzyme data.
  • VIRS offers a user-friendly and comprehensive solution for restriction mapping and analysis.