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Updated: Jun 21, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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Parallel gene cloning and protein production in multiple expression systems.

Hui-Min Wang1, Yan-Ping Shih, Su-Ming Hu

  • 1Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC.

Biotechnology Progress
|July 29, 2009
PubMed
Summary
This summary is machine-generated.

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Researchers developed versatile vectors for rapid recombinant protein production. This method enables simultaneous gene cloning and protein expression in both prokaryotic and eukaryotic systems, streamlining the search for optimal expression conditions.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Protein Expression

Background:

  • Optimizing recombinant protein production requires screening multiple expression systems.
  • Traditional methods involve significant cloning effort for each system.

Purpose of the Study:

  • To develop a streamlined approach for identifying optimal expression systems.
  • To enable parallel cloning and protein production in diverse systems.

Main Methods:

  • Construction of a set of vectors with identical restriction sites (5'-EcoRI/3'-XhoI).
  • Inclusion of sequences for protein tags and protease cleavage sites.
  • Parallel cloning of target genes into vectors for prokaryotic and eukaryotic systems.

Main Results:

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Related Experiment Videos

Last Updated: Jun 21, 2026

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Published on: June 27, 2017

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

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  • Successful parallel gene cloning into multiple expression vectors.
  • Facilitation of simultaneous recombinant protein production.
  • Demonstration of minimal cloning effort for system screening.

Conclusions:

  • The developed vectors enable efficient parallel screening of expression systems.
  • This approach accelerates the identification of optimal conditions for recombinant protein production.
  • Reduces labor and time in protein expression optimization.