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Published on: May 4, 2018
[Effects of Simvastatin on osteoblast activity of human periodontal ligament cells]
Fei Hu1, Xue-Yang Zhang, Chun-Xian Wang
1Dept. of Orthodontics, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
Objective:
To study the effects of Simvastatin on the osteoblast activity of human periodontal ligament (PDL) cells.
Methods:
The third passage human PDL were cultured in conditional mineralization medium with different concentrations of Simvastatin. The cells were divided into A group (0 mol/L), B group (1 x 10(-9) mol/L), C group (1 x 10(-8) mol/L), D group (1 x 10(-7) mol/L) and E group (1 x 10(-6) mol/L). Alkaline phosphatase (ALP) activity, osteopontin (OPN) and capability of mineralization were measured.
Results:
Differentiation osteoblast and mineralization of human PDL were improved in all treatment groups with different concentrations of Simvastatin (1 x 10(-9), 1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol/L). Compared with control group, statistically significant differences were found in 1 x 10(-8) mol/L, 1 x 10(-7) mol/L and 1 x 10(-6) mol/L groups (P<0.05). The maximum effect was observed at the concentration of 1 x 10(-7) mol/L.
Conclusion:
Optimal concentration of Simvastatin can improve the osteoblastic activity of human PDL.
