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Published on: July 25, 2017
[Statistical methods to optimize immobilization and bacteriocin production by Lactobacillus paracasei HD1.7]
Jianwei Chen1, Xiuliang Li, Bo Yin
1Education Department Key Microbiology Lab, College of Life Science, Heilongjiang University, Harbin 150080, China. cjw983617@yahoo.cn
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|July 30, 2009
Summary
Statistical optimization of immobilized Lactobacillus paracasei HD1.7 enhanced bacteriocin production. Immobilization using calcium alginate gel led to a 19% increase in total bacteriocin output.
Area of Science:
- Microbiology
- Biotechnology
- Bioprocess Engineering
Context:
- Bacteriocin production by Lactobacillus paracasei HD1.7 is crucial for antimicrobial applications.
- Immobilization techniques are explored to improve microbial production efficiency.
- Calcium alginate gel offers a suitable matrix for cell immobilization.
Purpose:
- To optimize bacteriocin production by Lactobacillus paracasei HD1.7 immobilized in calcium alginate gel.
- To identify key variables influencing bacteriocin yield using statistical design methods.
- To maximize bacteriocin production and cell cycle number through response surface methodology.
Summary:
- A fractional factorial design identified sodium alginate concentration, inoculation volume, and culture time as significant factors.
- Steepest ascent and Box-Behnken designs were employed to determine optimal conditions.
- Optimal conditions included 2.8% sodium alginate, 5% CaCl2, 4h immobilization, 1/20 cell density, 63h culture time, and 8.2% inoculation volume.
Impact:
- Optimized immobilization and fermentation conditions resulted in a 19% increase in total bacteriocin production.
- Immobilized beads demonstrated stability with minimal cell leakage, allowing for repeated use.
- This study provides a foundation for further research and extraction of bacteriocins.
