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Published on: September 10, 2016
Mechanisms involved in the immunostimulation by probiotic fermented milk
Carolina Maldonado Galdeano1, Alejandra de Moreno de Leblanc, Esteban Carmuega
1Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán (T4000ILC) Tucumán, Argentina.
The Journal of Dairy Research
|July 30, 2009
Summary
Probiotic fermented milk containing Lactobacillus casei DN114001 stimulates gut immunity. This enhances the non-specific barrier and innate immune response, promoting intestinal homeostasis.
Area of Science:
- Gastroenterology
- Immunology
- Microbiology
Background:
- The gut immune system's modulation by probiotics is not fully understood.
- Probiotics, like Lactobacillus casei DN114001, may influence gut immunity.
- Understanding these mechanisms is crucial for gut health.
Purpose of the Study:
- To investigate the effects of Lactobacillus casei DN114001 and its fermented milk on the gut immune system.
- To analyze the interaction of probiotics with intestinal cells and immune components.
- To assess the impact on innate and adaptive immune responses in the gut.
Main Methods:
- BALB/c mice were administered Lactobacillus casei DN114001 or probiotic fermented milk (PFM).
- Probiotic-intestinal cell interactions were visualized using electron and fluorescence microscopy.
- Immunological parameters were measured in intestinal tissue and cell supernatants, including cytokine release and immune cell populations.
Main Results:
- Lactobacillus casei DN114001 interacts with intestinal cells and gut-associated immune cells.
- PFM stimulated intestinal cells, increasing IL-6 release and activating macrophages.
- PFM enhanced populations of T lymphocytes and IgA+ B lymphocytes, indicating modulation of adaptive immunity.
Conclusions:
- Probiotic fermented milk stimulates mucosal immunity by reinforcing the non-specific barrier.
- It modulates the innate immune response and influences adaptive immunity in the gut.
- These effects contribute to maintaining intestinal homeostasis.
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