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Updated: Jun 21, 2026

Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization
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Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization

Published on: November 17, 2023

An improved method for constructing tissue microarrays from prostate needle biopsy specimens.

F McCarthy1, A Fletcher, N Dennis

  • 1Institute of Cancer Research, Male Urological Cancer Research Centre, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK. colin.cooper@icr.ac.uk

Journal of Clinical Pathology
|July 30, 2009
PubMed
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High-density tissue microarrays (TMAs) from prostate needle biopsies enable high-throughput analysis of diagnostic and prognostic biomarkers using immunohistochemical (IHC) and fluorescence in situ hybridisation (FISH) assays.

Area of Science:

  • Oncology
  • Pathology
  • Biomarker Research

Background:

  • Prostate cancer diagnosis relies on histopathology of needle biopsies, often the sole tissue source for biomarker analysis.
  • Existing methods for analyzing biomarkers in biopsy samples are limited in throughput.
  • There is a need for high-throughput methods for analyzing immunohistochemical (IHC) and fluorescence in situ hybridisation (FISH) markers in prostate biopsy tissues.

Purpose of the Study:

  • To develop and present methods for rapid and uniform construction of high-density biopsy tissue microarrays (TMAs).
  • To enable multiplex analysis of diagnostic and prognostic biomarkers in prostate needle biopsy specimens.

Main Methods:

  • Two novel methods were developed for constructing biopsy TMAs with 54–72 cores.

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Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking
05:49

Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking

Published on: October 10, 2019

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Last Updated: Jun 21, 2026

Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization
07:34

Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization

Published on: November 17, 2023

Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking
05:49

Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking

Published on: October 10, 2019

  • Tissue microarrays were constructed from prostate needle biopsy specimens from patients in active surveillance and radiotherapy trials.
  • Immunohistochemical (IHC) and fluorescence in situ hybridisation (FISH) techniques were employed to detect biomarker status.

    • Main Results:

    • Biopsy TMAs were successfully constructed from 102 active surveillance and 201 radiotherapy trial patients.
    • Cancer detection rates in biopsy TMA slices were 66% and 79%, respectively.
    • Multiplex IHC marker detection and TMPRSS2-ERG fusion status scoring via FISH were demonstrated on biopsy TMA slices.

    Conclusions:

    • Biopsy TMAs offer an effective platform for multiplex analysis of IHC and FISH markers.
    • This method facilitates the assessment of biomarkers as prognostic tools within clinical trials.
    • High-density biopsy TMAs improve the efficiency of biomarker analysis in prostate cancer research.