Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 21, 2026

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

An improved method for isolating Schwann cells from postnatal rat sciatic nerves.

Yujun Wei1, Jianli Zhou, Zhenghuan Zheng

  • 1Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China.

Cell and Tissue Research
|July 30, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collagen nanofiber reinforced alginate hydrogel tube microbioreactors for cell culture.

Frontiers in bioengineering and biotechnology·2026
Same author

The intravenous targeted nanopeptide, CAQK, is neuroprotective and improves motor function after spinal cord injury in rats.

The journal of trauma and acute care surgery·2026
Same author

Chemotherapeutic induction of cytosolic single-stranded DNA accumulation sensitizes triple-negative breast cancer to immunotherapy.

Journal for immunotherapy of cancer·2026
Same author

Fusion expression of carbohydrate-binding module and linker peptide improves the product specificity of chitosanase.

International journal of biological macromolecules·2026
Same author

Cross-modal training reduced susceptibility to the sound-induced flash illusion and generalized across stimulus onset asynchronies.

Psychological research·2026
Same author

Microbial community reshape and TTMP enhancement in daqu via targeted bioaugmentation of Bacillus velezensis and Bacillus licheniformis.

Food microbiology·2026
Same journal

Correction to: Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Deletion of CEACAM1 does not affect retinal and choroidal morphology or transcriptome.

Cell and tissue research·2026
Same journal

Cardiac α2δ1 C-terminal contributes to left atrial hypertrophy in chronic ischemic heart failure, in association with changes in membrane GluN1 and p-CAMKII/p-HDAC4 signaling.

Cell and tissue research·2026
Same journal

Gill ionocytes of the Lake Magadi tilapia (Oreochromis Alcolapia grahami), an extremophilic teleost native to a highly alkaline environment.

Cell and tissue research·2026
Same journal

Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Effects of gestational protein restriction on autophagy dynamics during odontogenesis.

Cell and tissue research·2026
See all related articles

This study presents a rapid, cost-effective method for purifying Schwann cells (SCs) from rat sciatic nerves, achieving over 99% purity. The technique combines cytosine-B-arabinoside hydrochloride (Ara-C) treatment and differential cell detachment to eliminate fibroblast contamination efficiently.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biotechnology

Background:

  • Schwann cell (SC) purification is challenging due to fibroblast contamination.
  • Existing methods are often costly, complex, or yield low purity.
  • Developing an efficient SC purification protocol is crucial for research and therapeutic applications.

Purpose of the Study:

  • To develop a rapid, efficient, and easily applicable method for obtaining highly purified SCs from newborn rat sciatic nerves.
  • To overcome the limitations of current SC purification techniques.
  • To achieve SC purity exceeding 99% while minimizing cost and complexity.

Main Methods:

  • A two-step purification process involving cytosine-B-arabinoside hydrochloride (Ara-C) treatment followed by differential cell detachment.

More Related Videos

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
08:57

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
09:09

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Published on: July 12, 2022

Related Experiment Videos

Last Updated: Jun 21, 2026

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
08:57

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

Published on: February 10, 2023

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
09:09

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Published on: July 12, 2022

  • Initial culture of SCs from rat sciatic nerves.
  • Treatment with Ara-C to eliminate fibroblasts, followed by culture with human heregulin1-beta1 extracellular domain (HRG1-beta1 ECD).
  • Selective detachment of SCs using trypsin, separating them from remaining fibroblasts.

    • Main Results:

    • Achieved SC purity of over 99%, confirmed by morphology and S-100 immunostaining.
    • The combined method effectively eliminated fibroblast contamination.
    • The entire procedure, from primary culture to confluent purified SCs, takes approximately 10 days.
    • Purified SCs exhibited characteristic spindle-shaped morphology and aligned in fascicles.

    Conclusions:

    • The developed protocol offers an efficient and rapid method for high-purity Schwann cell isolation.
    • This technique provides a valuable alternative for peripheral nerve regeneration studies.
    • The method has potential for generating sufficient SCs for in vitro artificial nerve scaffold construction.