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[Determination of quercitrin in Polygonum capitatum and relinqing granules by HPLC]
Yu Xie1, Liyan Zhang, Bin Liang
1Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Objective:
To establish an HPLC method for determination the contents of quercitrin in Polygonum capitatum and Relinqing granules.
Method:
The samples were analyzed on a Diamonsil C18 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of methanol-1% HAc-THF. The flow rate was 1.0 mL x min-m. The detection wavelength was set at 258 nm and the column temperature was 25 degrees C.
Result:
The calibration curve was linear in the range of 0. 081 64-0.4084 microg (r = 0.99997). The average recovery rate of quercitrin in P. capitatum was 102.3% (RSD 0.99%), and was 102.7% (RSD 2.2%) in Relinqing granules.
Conclusion:
The method is reliable and specific with good repeatability, and can be used for the quality control of P. capitatum and Relinqing granules. It can provide a science bases for the planting of polygonum capitatum.
