Related Experiment Video
Updated: Jun 21, 2026
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Published on: June 6, 2022
Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system
Meng Wu1, Shunyou Long, Anthony G Frutos
1Department of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.
Abstract:
The Epic system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.
