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Published on: September 6, 2024
Effect of rapeseed peptide on DNA damage and apoptosis in Hela cells
Zhaohui Xue1, Zhiwei Liu, Moucheng Wu
1School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, PR China.
Abstract:
Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640 mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay. We observed a dose-dependent increase in cytotoxicity induced by RSP at 20-640 mg/L. After 4 days of 320 mg/L RSP treatment, typical apoptotic changes were observed by transmission electron microscopy (TEM). Using the comet assay, we found dramatic comet tails, indicating DNA damage by RSP (20-640 mg/L). Moreover, RSP treatment caused inhibition of Hela cell growth, with cycle arrest in the S phase and apoptosis induction. Taken together, the results suggested that rapeseed peptide could be a potential antitumor compound with an apoptotic mode of action.
Insights
Rapeseed peptide (RSP) shows anticancer potential by inhibiting human cervical carcinoma (Hela) cell proliferation and inducing apoptosis. This study highlights RSP as a promising antitumor compound.
Area of Science:
- Biochemistry
- Molecular Biology
- Cancer Research
Background:
- Rapeseed peptide (RSP), derived from rapeseed protein, exhibits anticancer properties.
- Cervical carcinoma, specifically Hela cells, remains a significant health concern requiring novel therapeutic strategies.
Purpose of the Study:
- To investigate the anticancer effects of RSP on Hela cells.
- To elucidate the mechanisms underlying RSP's antitumor activity, including effects on proliferation, DNA damage, cell cycle, and apoptosis.
Main Methods:
- MTT assay for cell proliferation assessment.
- Transmission electron microscopy (TEM) for morphological changes.
- Comet assay for DNA damage evaluation.
- Cell cycle analysis.
Main Results:
- RSP significantly inhibited Hela cell proliferation in a dose-dependent manner.
- RSP induced significant DNA damage and characteristic apoptotic morphological changes in Hela cells.
- RSP treatment led to S-phase cell cycle arrest and apoptosis induction.
Conclusions:
- Rapeseed peptide demonstrates potent antitumor activity against Hela cells.
- The mechanism of action involves inducing DNA damage, cell cycle arrest, and apoptosis.
- RSP is a potential candidate for developing novel anticancer therapies.
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