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Updated: Jun 21, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Determining seminal plasma human immunodeficiency virus type 1 load in the context of efficient highly active
Christophe Pasquier1, Karine Sauné, Stéphanie Raymond
1Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, TSA40031, 330 avenue de Grande Bretagne, Toulouse F-31059, France. pasquier.c@chu-toulouse.fr
Abstract:
The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to measure the HIV-1 load in semen plasma samples. HIV-1 was detected in 13.4% of the semen samples processed at our MAP center. Of the eight patients having a detectable semen HIV-1 load, five had no detectable virus in their blood plasma. This highlights the residual risk of HIV-1 transmission during unprotected intercourse and raises the question of the possible consequences of ineffective highly active antiretroviral therapy in the genital tract.
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