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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

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Related Experiment Video

Updated: Jun 21, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

PUMA, a potent killer with or without p53.

J Yu1, L Zhang

  • 1Department of Pathology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.

Oncogene
|July 31, 2009
PubMed
Summary
This summary is machine-generated.

PUMA (p53 upregulated modulator of apoptosis) is a key protein mediating cell death. Its regulation is crucial for preventing cancer and tissue damage, making it a significant therapeutic target.

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

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Last Updated: Jun 21, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
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Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • PUMA (p53 upregulated modulator of apoptosis) is a BH3-only Bcl-2 family member.
  • It mediates p53-dependent and -independent apoptosis in response to diverse cellular stressors.
  • PUMA acts as a proximal signaling molecule, regulating mitochondrial apoptosis pathways.

Purpose of the Study:

  • To elucidate the role of PUMA in apoptosis.
  • To investigate PUMA's mechanism of action in transducing death signals.
  • To explore PUMA's therapeutic potential in cancer and tissue injury.

Main Methods:

  • The study focuses on the molecular mechanisms of PUMA function.
  • Analysis of PUMA's interactions with other Bcl-2 family members.
  • Investigation of PUMA's role in cellular responses to various stimuli.

Main Results:

  • PUMA antagonizes antiapoptotic Bcl-2 proteins, inducing mitochondrial dysfunction and caspase activation.
  • PUMA ablation results in apoptosis deficiency, linked to cancer development and therapeutic resistance.
  • Elevated PUMA expression sensitizes cancer cells to chemotherapy and radiotherapy.

Conclusions:

  • PUMA is a critical sensor of cell death stimuli.
  • Modulating PUMA offers therapeutic strategies for cancer and degenerative diseases.
  • PUMA represents a promising drug target for managing cell death-related pathologies.