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A combined physical and genetic map of Pseudomonas aeruginosa PAO
E Ratnaningsih1, S Dharmsthiti, V Krishnapillai
1Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Journal of General Microbiology
|December 1, 1990
Abstract:
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.