[Cell nucleus analyses on the heterogenicity, proliferation and metastasis of malignant tumors]

The heterogeneity of primary and metastatic tumors was studied on the basis of their nuclear images. Feulgen-stained tissue sections of invasive ductal carcinomas of the breast (84 primaries, 15 lymph node metastases), adenocarcinomas of the stomach (30 primaries) and colorectal adenocarcinomas (30 primaries, 10 lymph node metastases) were investigated by high resolution image analysis. At least 150 tumour cell nuclei as well as 50 diploid reference cells were measured in each case. By means of multivariate statistical analysis significant differences could be found between primary tumors with and without lymph node involvement or distant metastases. In contrast, in a majority of the cases primary tumors and their metastases were largely identical with regard to the nuclear image and the DNA content. Features with high discriminant power for differentiation of metastasising tumors and malignant tumors without metastatic spread were the nuclear area, the number of coarse chromatin particles and other descriptors of the chromatin structure. The DNA content as a useful tool for the prediction of prognosis was of minor importance for the prediction of the metastatic phenotype.