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Updated: Jun 21, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
Multiplex single-nucleotide polymorphism typing by nanoparticle-coupled DNA-templated reactions
Xuejia Xue1, Wei Xu, Feng Wang
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
Abstract:
A novel chip-based detection approach for single-nucleotide polymorphism (SNP) typing based on nanoparticle-coupled DNA-templated ligation reactions is reported. In contrast to conventional methods or recently developed techniques, this approach does not need costly instrumentation and complex stringency washing processes and offers both rapid multiplex SNP detection capability and ultrahigh sensitivity. The ability of the approach to quickly identify the precise location of the single-base mismatch may provide a time-efficient method for high-throughput multiplex SNP genotyping.
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