Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Malaria01:29

Malaria

Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-malarial contact dependent blocking of transmission of Plasmodium vivax by Anopheles darlingi mosquito vector.

PLoS pathogens·2026
Same author

Rising burden of Plasmodium vivax in Mizoram: an emerging threat to malaria elimination in Northeast India.

BMC infectious diseases·2026
Same author

Genetic diversity and gut microbiome of Anopheles mosquitoes in Tamil Nadu by using COI DNA barcoding and 16S rRNA metagenomics.

Scientific reports·2026
Same author

Anopheles maculatus s.l as potential malaria vectors with opportunistic feeding in Meghalaya, Northeast India.

Scientific reports·2026
Same author

Relevance of the antibody Fc fragment and epitope valency in protection against malaria sporozoites.

EMBO reports·2026
Same author

Impact of projected climate and socioeconomic scenarios on state-wise annual dengue incidence in India using ensemble models.

PLoS neglected tropical diseases·2026

Related Experiment Video

Updated: Jun 21, 2026

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
09:03

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

Published on: June 10, 2015

Plasmodium falciparum enolase: stage-specific expression and sub-cellular localization.

Ipsita Pal Bhowmick1, Nirbhay Kumar, Shobhona Sharma

  • 1Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai-400005, India. ipsitapb@gmail.com

Malaria Journal
|August 1, 2009
PubMed
Summary

Plasmodium falciparum enolase, a potential malaria vaccine target, exhibits diverse cellular locations throughout the parasite lifecycle. This suggests enolase performs multiple non-glycolytic functions beyond its known role in glycolysis.

More Related Videos

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

Related Experiment Videos

Last Updated: Jun 21, 2026

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
09:03

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

Published on: June 10, 2015

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

Area of Science:

  • Malariology
  • Parasitology
  • Molecular Biology

Background:

  • Previous studies indicated Plasmodium falciparum enolase vaccination offers partial protection against malaria in mice.
  • Growing evidence suggests enolases have non-glycolytic functions in pathogens.
  • Investigating stage-specific expression and localization can reveal protein moonlighting functions.

Purpose of the Study:

  • To determine the sub-cellular localization and stage-specific expression of Plasmodium falciparum enolase.
  • To explore potential non-glycolytic roles of enolase in the parasite lifecycle.

Main Methods:

  • Immunofluorescence assay
  • Immuno-gold electron microscopy
  • Western blotting

Main Results:

  • Enolase was detected throughout all examined parasite life stages.
  • In asexual stages, enolase was primarily in the soluble fraction (>85-90%).
  • In sexual stages, enolase was mainly associated with the particulate fraction.
  • Enolase localized to the cytosol, nucleus, food vacuole, cytoskeleton, and plasma membrane.

Conclusions:

  • Diverse enolase localization implies roles beyond glycolysis, such as merozoite surface association for red blood cell invasion.
  • Vacuolar enolase may be involved in food vacuole formation or development.
  • Nuclear enolase might play a role in transcription.