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Lagging Strand Synthesis

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
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Related Experiment Video

Updated: Jun 21, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Primer fabrication using polymerase mediated oligonucleotide synthesis.

Murray J Cairns¹, Torsten Thomas, Carolina E Beltran

¹Schizophrenia Research Institute, Sydney, NSW 2006, Australia. murray.cairns@newcastle.edu.au

|August 1, 2009

Summary

Polymerase mediated oligonucleotide synthesis (PMOS) offers a cost-effective and rapid method for generating gene-specific primers. This novel approach utilizes DNA polymerase to synthesize primers, potentially replacing traditional, expensive custom synthesis methods.

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Chemical Triphosphorylation of Oligonucleotides

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Last Updated: Jun 21, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Chemical Triphosphorylation of Oligonucleotides

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Custom solid-phase oligonucleotide synthesis is crucial for genomics but is expensive, time-consuming, and wasteful.
Existing methods for producing oligonucleotide primers do not meet the demand for speed and cost-efficiency.

Purpose of the Study:

To introduce and validate a novel method for oligonucleotide synthesis using DNA polymerase.
To demonstrate the efficiency and applicability of polymerase mediated oligonucleotide synthesis (PMOS) for generating gene-specific primers.

Main Methods:

Utilized a DNA polymerase to increase oligonucleotide hybridization affinity using a template for DNA synthesis.
Developed a universal 768-member oligonucleotide library (UniSeq) for PMOS.
Evaluated PMOS performance in Polymerase Chain Reaction (PCR) and DNA sequencing reactions.

Main Results:

PMOS successfully generated specific 11-mer primers from a small precursor library.
The primers produced via PMOS performed comparably to those from custom synthesis in PCR and sequencing.
Demonstrated the ability to generate primers directly in the laboratory for various DNA polymerase chemistries.

Conclusions:

PMOS is a broadly applicable and novel system for oligonucleotide synthesis.
This method has the potential to replace conventional, expensive oligonucleotide synthesis.
PMOS offers a faster, more cost-effective, and less wasteful alternative for primer generation.