Related Experiment Video
Updated: Jun 21, 2026
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Published on: February 8, 2021
Performance characteristics of beta-2-microglobulin measurements on a Beckman Immage analyzer with the DakoCytomation
Barun K De1, Ernest Jimenez, Soumo De
1Department of Pathology, University of Arizona Health Sciences Center, Tucson, AZ 85724-5059, USA. bkde@email.arizona.edu
Objective:
Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer.
Design And Methods:
The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry.
Results:
The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope=1.009, r=0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L.
Conclusion:
The DakoCytomation reagent is acceptable to measure serum beta2M.
