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Updated: Jun 21, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat
Gordana S Timotijević1, Mira Dj Milisavljević, Svetlana R Radović
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia. timotijevic@imgge.bg.ac.rs
Abstract:
The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.
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