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Updated: Jun 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Correction for interference by test samples in high-throughput assays.

Adam B Shapiro¹, Grant K Walkup, Thomas A Keating

¹AstraZeneca R&D Boston, Waltham, Massachusetts 02451, USA. adam.shapiro@astrazeneca.com

Journal of Biomolecular Screening

|August 1, 2009

Summary

This summary is machine-generated.

A new method corrects for sample interference in high-throughput biochemical assays. This artifact correction improves optical measurements by accounting for sample-specific detection issues in multiwell plates.

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Area of Science:

Biochemistry
Assay Development
High-Throughput Screening

Background:

High-throughput biochemical assays commonly use optical detection methods (absorbance, fluorescence, luminescence, scintillation counting) in multiwell plates.
Test samples can interfere with optical measurements due to light absorption, fluorescence quenching, sample fluorescence, reagent interactions, or meniscus effects.
Accurate measurement of sample activity is crucial for drug discovery and biochemical research.

Purpose of the Study:

To present a simple, well-by-well method for correcting optical detection interference caused by test samples in biochemical assays.
To enable more accurate activity measurements in high-throughput screening and potency determination.
To provide a broadly applicable correction procedure for various optical detection modalities.

Main Methods:

A separate artifact assay plate is used to measure the interference caused by each test sample.
Arithmetic correction is applied to the corresponding well in the primary activity assay plate.
The method is validated for both single-point screening and serial dilution potency measurements.

Main Results:

The described method effectively corrects for various types of optical detection interference.
Accurate measurements are achieved even in the presence of interfering sample properties.
The correction procedure is straightforward to implement in standard laboratory workflows.

Conclusions:

This artifact correction method enhances the reliability of optical measurements in high-throughput biochemical assays.
It offers a practical solution for mitigating sample-induced detection interference.
The technique supports more robust screening and potency assays, crucial for advancing biochemical research and drug discovery.