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Related Experiment Video

Updated: Jun 21, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
13:45

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Human papillomavirus genotyping using an automated film-based chip array.

Maria Erali1, David C Pattison, Carl T Wittwer

  • 1ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA. maria.erali@aruplab.com

The Journal of Molecular Diagnostics : JMD
|August 1, 2009
PubMed
Summary

The INFINITI HPV-QUAD assay shows promise for human papillomavirus (HPV) genotyping. This automated system offers user-friendly identification of distinct HPV genotypes, warranting further clinical utility studies.

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Area of Science:

  • Molecular diagnostics
  • Virology
  • Genotyping technologies

Background:

  • Human papillomavirus (HPV) is a major cause of cervical cancer.
  • Accurate HPV detection and genotyping are crucial for effective patient management.
  • Existing HPV tests have limitations in specificity and type identification.

Purpose of the Study:

  • To evaluate the analytical performance of the commercially available INFINITI HPV-QUAD assay.
  • To compare the HPV-QUAD assay with the digene High-Risk HPV hc2 Test (HC2) using liquid cervical cytology specimens.
  • To assess the HPV-QUAD assay's ability to identify distinct HPV genotypes.

Main Methods:

  • Multiplex PCR followed by automated primer extension, hybridization, and detection.
  • Evaluation using liquid cervical cytology specimens in Surepath and PreservCyt media.

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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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  • Comparison with the digene High-Risk HPV hc2 Test (HC2).
  • Real-time PCR and sequence analysis for discrepant results.

    • Main Results:

    • Overall concordance between HPV-QUAD and HC2 was 83% after resolving indeterminate results.
    • Discrepancies included false positives in HC2 and false negatives in HPV-QUAD, often due to cross-reactivity or low viral load.
    • Commonly identified HPV types included HPV16, HPV18, HPV52/58, and HPV39/56.
    • The HPV-QUAD assay demonstrated user-friendliness and automation for distinct HPV genotype identification.

    Conclusions:

    • The INFINITI HPV-QUAD assay is a user-friendly, automated system for identifying distinct HPV genotypes.
    • Analytical performance suggests potential for clinical utility in HPV detection and genotyping.
    • Further studies are warranted to assess the clinical utility of the HPV-QUAD platform.