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[Tissue culture course of a human hepatoma cell line]
Y Kusaka1, T Tokiwa, N A Monterio
1Division of Pathology, Okayama University Medical School, Japan.
Human Cell
|March 1, 1990
Abstract:
A cell line, HuH-33 was cultured in vitro from a patient with hepatocellular carcinoma. This cell line has been in continuous culture over 12 month period with slow growth potential. HuH-33 was composed spindle-or polygonal-shaped cells as a major population. Chromosome number of the cells were widely distributed even in the primary culture. HuH-33 was transplantable into nude mice and secreted alpha-fetoprotein, albumin, beta 2 microglobulin, ferritin and tissue polypeptide antigen.