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Related Experiment Videos

Heparin, monocytes, and procoagulant activity.

R Abbate1, A M Gori, P A Modesti

  • 1Clinica Medica I, University of Florence, Italy.

Haemostasis
|January 1, 1990
PubMed
Summary

Monocytes bind heparin specifically and reversibly, with potential for internalization. Cell stimulation increases heparin binding sites, impacting monocyte tissue factor expression and reducing procoagulant potential.

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Area of Science:

  • Immunology
  • Hematology
  • Biochemistry

Background:

  • Monocytes play a crucial role in immune responses and hemostasis.
  • Heparin is a widely used anticoagulant with known interactions with various cell types.
  • Understanding monocyte-heparin interactions is vital for comprehending anticoagulation mechanisms and immune modulation.

Purpose of the Study:

  • To investigate the specific binding characteristics of heparin to human monocytes.
  • To determine the effect of cell stimulation on heparin binding.
  • To elucidate the impact of heparin-monocyte interaction on tissue factor expression and procoagulant activity.

Main Methods:

  • Quantification of heparin binding sites on monocytes using radiolabeled heparin.
  • Assessment of heparin internalization by monocytes.

Related Experiment Videos

  • Evaluation of monocyte activation markers and tissue factor expression following heparin exposure.
  • Stimulation of monocytes with calcium ionophore, lipopolysaccharide, and interleukin-2.

    • Main Results:

    • Monocytes exhibit specific, rapid, reversible, and saturable binding of heparin.
    • Approximately 5.5 x 10^6 binding sites per monocyte were identified with a dissociation constant of 330 nmol/l.
    • 40-50% of cell-bound heparin can be internalized by monocytes.
    • Cell stimulation significantly increases the availability of heparin binding sites.
    • Heparin binding reversibly decreases surface tissue factor expression on monocytes, thereby reducing their procoagulant potential.

    Conclusions:

    • Monocytes possess a significant capacity for specific heparin binding and internalization.
    • Monocyte activation enhances heparin binding, suggesting a dynamic interaction.
    • Heparin interaction with monocytes modulates their procoagulant activity by downregulating tissue factor expression.