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Related Experiment Video

Updated: Jun 21, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Nanostructured zinc oxide platform for mycotoxin detection.

Anees A Ansari1, Ajeet Kaushik, Pratima R Solanki

  • 1Department of Science & Technology Centre on Biomolecular Electronics, New Delhi, India. aneesaansari@gmail.com

Bioelectrochemistry (Amsterdam, Netherlands)
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

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This study developed a nanostructured zinc oxide (Nano-ZnO) sensor for detecting ochratoxin-A (OTA). The novel biosensor demonstrates high sensitivity and a low detection limit for accurate OTA analysis.

Area of Science:

  • Materials Science
  • Electrochemistry
  • Biosensing

Background:

  • Ochratoxin-A (OTA) is a harmful mycotoxin requiring sensitive detection methods.
  • Nanostructured materials offer unique properties for biosensor development.
  • Zinc oxide (ZnO) nanostructures are promising for electrochemical applications.

Purpose of the Study:

  • To develop a novel electrochemical biosensor for ochratoxin-A (OTA) detection.
  • To utilize nanostructured zinc oxide (Nano-ZnO) for enhanced sensor performance.
  • To co-immobilize rabbit-immunoglobulin antibodies (r-IgGs) and bovine serum albumin (BSA) on Nano-ZnO for specific OTA recognition.

Main Methods:

  • Deposition of nanostructured zinc oxide (Nano-ZnO) film onto indium-tin-oxide (ITO) glass.
  • Characterization of Nano-ZnO/ITO electrode using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM).

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Aerosol-assisted Chemical Vapor Deposition of Metal Oxide Structures: Zinc Oxide Rods

Published on: September 14, 2017

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Last Updated: Jun 21, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Aerosol-assisted Chemical Vapor Deposition of Metal Oxide Structures: Zinc Oxide Rods
06:39

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Published on: September 14, 2017

  • Fabrication of the BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode and electrochemical impedance spectroscopy (EIS) analysis for OTA detection.

    • Main Results:

    • Formation of Nano-ZnO with an average particle size of approximately 5.0 nm confirmed by XRD.
    • Successful characterization of the Nano-ZnO/ITO electrode and the BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode.
    • Electrochemical impedimetric response showed linearity from 0.006-0.01 nM/dm³, a detection limit of 0.006 nM/dm³, a response time of 25s, and a sensitivity of 189 Ω/nM/dm²cm⁻² (R²=0.997).

    Conclusions:

    • The developed BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode is effective for sensitive and rapid ochratoxin-A detection.
    • Nano-ZnO serves as an excellent platform for co-immobilization, enhancing biosensor performance.
    • This approach offers a promising method for ochratoxin-A monitoring in various samples.