T Cell Activation and Clonal Selection
Cells of the Adaptive Immune Response
T Cell Types and Functions
Lineage Commitment
Special Features of Adaptive Immunity
Cell-mediated Immune Responses
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Updated: Jun 21, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Terry B Strom1, Maria Koulmanda
1Departments of Medicine and Surgery, Harvard Medical School, Transplant Institute, Division of Immunology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. tstrom@bidmc.harvard.edu
Resting CD4(+) T cells differentiate into distinct phenotypes like Th1 and Th2 cells, which are terminally differentiated. However, regulatory T cells (Tregs) and Th17 cells are not terminally differentiated and adapt to their inflammatory environment.
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