Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 21, 2026

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
11:22

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Published on: December 22, 2014

Genetic address book for retinal cell types.

Sandra Siegert1, Brigitte Gross Scherf, Karina Del Punta

  • 1Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Nature Neuroscience
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing mechanobiology from single molecules to complex cellular systems.

Nature nanotechnology·2026
Same author

Revisiting retinal and macular degeneration in the genomics era.

Nature reviews. Genetics·2026
Same author

Improving positively tuned voltage indicators for faster kinetics and higher contrast.

bioRxiv : the preprint server for biology·2026
Same author

Cell-type-targeted mitochondrial transplantation rescues cell degeneration.

Nature·2026
Same author

Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.

Neuron·2026
Same author

Artificial intelligence-driven whole-brain cell mapping with highly multiplexed in situ hybridization.

Neuron·2026
Same journal

Glycosylation in Alzheimer's disease.

Nature neuroscience·2026
Same journal

Neuropixels harness the light.

Nature neuroscience·2026
Same journal

Clarity in clearance pathways.

Nature neuroscience·2026
Same journal

Hypothalamic specification in a dish.

Nature neuroscience·2026
Same journal

Author Correction: A route for cerebrospinal fluid flow through leptomeningeal arterial-venous overlaps enables macromolecule and fluid shunting.

Nature neuroscience·2026
Same journal

Author Correction: Prefrontal engrams of long-term fear memory perpetuate pain perception.

Nature neuroscience·2026
See all related articles

Researchers identified new transgenic mouse lines that label specific retinal neurons. This advance aids in studying the brain's complex circuits, particularly for night vision and directional selectivity.

Area of Science:

  • Neuroscience
  • Genetics
  • Ophthalmology

Background:

  • The mammalian brain comprises thousands of neuronal cell types organized into distinct circuits for complex computations.
  • Dissecting functional components of these circuits requires tools like transgenic mouse lines that selectively mark cell types.

Purpose of the Study:

  • To screen for cell type-specific green fluorescent protein expression in the retina using BAC transgenic mice.
  • To identify mouse lines that selectively label inhibitory cell types within the night vision and directional selective circuits.
  • To assess the utility of these lines for studying retinal circuitry development and function.

Main Methods:

  • Conducted a screen of BAC transgenic mouse lines from the GENSAT project for cell type-specific green fluorescent protein expression in the retina.

More Related Videos

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Related Experiment Videos

Last Updated: Jun 21, 2026

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
11:22

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Published on: December 22, 2014

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

  • Quantified stratification patterns of labeled cell types to predict synaptic connectivity.
  • Utilized identified lines for targeted recordings and imaging in developing and mature retinal circuits.

    • Main Results:

    • Identified transgenic mouse lines with selective labeling of inhibitory cell types in the night vision and directional selective retinal circuits.
    • Stratification patterns predicted potential synaptic connections between different labeled cell types.
    • Several identified lines facilitated targeted recordings and imaging of specific retinal cell types.

    Conclusions:

    • The developed transgenic mouse lines are valuable tools for dissecting retinal neuronal circuitry.
    • The stratification-based screening approach shows potential for characterizing neuronal circuitry in other layered brain structures, like the neocortex.