Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Deconvolution01:20

Deconvolution

Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CK2 phosphorylates TNFAIP1 to affect its subcellular localization and interaction with PCNA.

Molecular biology reports·2009
Same author

Reamed and unreamed intramedullary nailing for the treatment of open and closed tibial fractures: a subgroup analysis of randomised trials.

International orthopaedics·2009
Same author

Selective COX-2 inhibitor versus nonselective COX-1 and COX-2 inhibitor in the prevention of heterotopic ossification after total hip arthroplasty: a meta-analysis of randomised trials.

International orthopaedics·2009
Same author

[Study on evaluating sex determining region of the Y as an engrafting track of BMSCs transplantation for repairing osteonecrosis of the femoral head of rabbit].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2009
Same author

Positive association between benign familial infantile convulsions and LGI4.

Brain & development·2009
Same author

Catalytic enantioselective synthesis of chiral phthalides by efficient reductive cyclization of 2-acylarylcarboxylates under aqueous transfer hydrogenation conditions.

Organic letters·2009
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

Blind deconvolution for thin-layered confocal imaging.

Praveen Pankajakshan1, Bo Zhang, Laure Blanc-Féraud

  • 1Ariana Project-team, INRIA/CNRS, 2004 Route des Lucioles, BP 93, 06902 Sophia-Antipolis Cedex, France. ppankaja@sophia.inria.fr

Applied Optics
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

We developed a new algorithm to accurately estimate the point-spread function (PSF) of confocal laser scanning microscopes. This method improves deconvolution results on real data compared to theoretical models.

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Analyzing Dendritic Morphology in Columns and Layers
08:41

Analyzing Dendritic Morphology in Columns and Layers

Published on: March 23, 2017

Related Experiment Videos

Last Updated: Jun 21, 2026

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Analyzing Dendritic Morphology in Columns and Layers
08:41

Analyzing Dendritic Morphology in Columns and Layers

Published on: March 23, 2017

Area of Science:

  • Microscopy
  • Image Processing
  • Computational Science

Background:

  • Confocal laser scanning microscopy (CLSM) is crucial for high-resolution imaging.
  • Accurate point-spread function (PSF) estimation is vital for effective image deconvolution.
  • Existing PSF estimation methods may have limitations in accuracy and convergence.

Purpose of the Study:

  • To propose an alternate minimization algorithm for PSF estimation in CLSM.
  • To simultaneously estimate the specimen fluorescence distribution.
  • To enhance deconvolution performance using an accurate PSF model.

Main Methods:

  • Developed an alternate minimization algorithm for PSF estimation.
  • Employed a 3D separable Gaussian model to constrain the PSF solution space.
  • Incorporated a specimen constraint to stabilize and ensure algorithm convergence.

Main Results:

  • Simulations demonstrated high accuracy in PSF estimation.
  • Real data deconvolution showed superior results compared to using a full theoretical PSF model.
  • The proposed algorithm effectively estimates both PSF and specimen distribution.

Conclusions:

  • The proposed alternate minimization algorithm provides accurate PSF estimation for CLSM.
  • This method offers improved deconvolution performance over theoretical PSF models.
  • The algorithm's constraints enhance stability and convergence for practical applications.