[High velocity mechanical injection of foreign DNA into fish oocytes]

High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.