Blood donor screening for parvovirus B19
B J Cohen1, A M Field, S Gudnadottir
1Virus Reference Laboratory, Central Public Health Laboratory, London, U.K.
Journal of Virological Methods
|December 1, 1990
Summary
Screening 24,000 blood donors identified one parvovirus B19 (B19) positive donation. This B19 positive material was recovered for use as a viral antigen in diagnostic serology.
Area of Science:
- Virology
- Immunology
- Public Health
Background:
- Parvovirus B19 (B19) poses a risk in transfusion medicine.
- Diagnostic serology requires reliable viral antigen sources.
Purpose of the Study:
- To screen blood donors for parvovirus B19.
- To obtain a B19 positive donation for viral antigen isolation.
Main Methods:
- Blood donor screening using counter current immunoelectrophoresis.
- Plasma pooling of positive donations.
- Acid dissociation technique for antigen recovery.
Main Results:
- One out of 24,000 donors tested positive for B19 antigen.
- Over 100 ml of B19 containing material was obtained.
- B19 antigen was successfully recovered from immune complexes in a plasma pool.
Conclusions:
- Blood donor screening can identify parvovirus B19 positive individuals.
- Recovered B19 antigen can be utilized for diagnostic assay development.
- Antigen recovery from plasma pools is feasible.
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