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[Effects of fluid shear stress time on carbonic anhydrase in rat osteoclasts]
Bao-min Zhu1, Qing-hong Zhang, Wei-qian Wang
1Department of Prosthetics, Hangzhou Stomatological Hospital, Hangzhou 310006, China.
Purpose:
To observe the effects of fluid shear stress on carbonic anhydrase II in rat osteoclasts.
Methods:
Osteoclasts were subject to fluid shear stress for 0,15,30,60 and 120 minutes respectively, which was 2.9dyne/cm(2). mRNA level of carbonic anhydrase II was detected through real-time fluorescent quantitation PCR. The data were analyzed with SPSS 12.0 software package.
Results:
mRNA level of carbonic anhydrase II in control group, 15 minutes,30 minutes,60 minutes and 120 minutes group was 7.88+/-0.09, 11.14+/-0.12, 15.83+/-0.18, 1.94+/-0.02, and 1.37+/-0.01(E+5 copy number), respectively. There was significant difference between any two groups (P<0.05).
Conclusions:
In this present study, mRNA level of carbonic anhydrase II has an increasing tendency with various action times of fluid shear stress, it reaches to a peak value at 30 minutes. We speculated that there might be a certain time-effect relation between fluid shear stress and mRNA level of carbonic anhydrase II, but this mechanism need be further studied.
