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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 21, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

A designed peptide chip: protein fingerprinting technology with a dry peptide array and statistical data mining.

Kenji Usui1, Kin-ya Tomizaki, Hisakazu Mihara

  • 1Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|August 4, 2009
PubMed
Summary

This study introduces a novel dry peptide microarray format for protein fingerprinting. This method enables high-throughput quantitative analysis of targeted proteins within complex biological systems.

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Last Updated: Jun 21, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
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Published on: April 17, 2012

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Microarray technology offers a high-throughput method for studying protein networks.
  • Protein-detecting microarrays utilize immobilized agents to capture and detect protein interactions.
  • Existing peptide microarray formats have limitations in usability and quantitative analysis.

Purpose of the Study:

  • To extend the usability of peptide microarrays.
  • To introduce a novel dry peptide microarray format.
  • To develop a statistical technique for quantitative analysis of targeted proteins.

Main Methods:

  • Development of a novel dry peptide microarray format.
  • Generation of protein fingerprint (PFP) data sets using the novel microarray.
  • Application of a statistical PFP data manipulation technique for quantitative analysis.

Main Results:

  • The novel dry peptide microarray format provides a new approach for protein analysis.
  • The statistical technique allows for quantitative assessment of targeted proteins.
  • The developed method enhances the utility of peptide microarrays in biological research.

Conclusions:

  • The novel dry peptide microarray format and statistical analysis technique offer a powerful tool for proteomics research.
  • This approach facilitates high-throughput, quantitative analysis of protein interactions.
  • The findings contribute to advancing the application of microarray technologies in understanding cellular protein networks.