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Published on: December 15, 2017
Application of microbioreactors in fermentation process development: a review.
Daniel Schäpper1, Muhd Nazrul Hisham Zainal Alam, Nicolas Szita
1Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Lyngby, Denmark.
Analytical and Bioanalytical Chemistry
|August 4, 2009
Summary
Microbioreactors offer advanced control and data for high-throughput strain screening in biotechnology. This technology improves upon traditional methods, enabling more reliable results for industrial applications.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Microbial Cultivation
Background:
- Biotechnology process development requires extensive strain testing and improvement for enhanced yields and productivity.
- Current high-throughput screening methods using shake flasks or microtiter plates suffer from low data density and poor control over cultivation conditions.
Purpose of the Study:
- To review current microbioreactor technology.
- To analyze the industrial applicability of microbioreactors.
- To present future research directions in microbioreactor technology.
Main Methods:
- Analysis of existing microbioreactor systems and their features.
- Evaluation of microbioreactors for high-throughput screening and process development.
- Comparison of microbioreactors with traditional shake flask and microtiter plate methods.
Main Results:
- Microbioreactors provide superior control and flexibility compared to traditional methods.
- They offer high data density with online cultivation data, crucial for process optimization.
- Microbioreactors enable results more comparable to large-scale fermentations.
Conclusions:
- Microbioreactors represent a significant advancement for strain development in biotechnology.
- Their controllability, automation potential, and data richness enhance industrial applicability.
- Further research should focus on optimizing microbioreactor designs and applications for large-scale bioprocesses.
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