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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Microbes in Food Production01:29

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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Application of microbioreactors in fermentation process development: a review.

Daniel Schäpper1, Muhd Nazrul Hisham Zainal Alam, Nicolas Szita

  • 1Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Lyngby, Denmark.

Analytical and Bioanalytical Chemistry
|August 4, 2009
PubMed
Summary

Microbioreactors offer advanced control and data for high-throughput strain screening in biotechnology. This technology improves upon traditional methods, enabling more reliable results for industrial applications.

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Microbial Cultivation

Background:

  • Biotechnology process development requires extensive strain testing and improvement for enhanced yields and productivity.
  • Current high-throughput screening methods using shake flasks or microtiter plates suffer from low data density and poor control over cultivation conditions.

Purpose of the Study:

  • To review current microbioreactor technology.
  • To analyze the industrial applicability of microbioreactors.
  • To present future research directions in microbioreactor technology.

Main Methods:

  • Analysis of existing microbioreactor systems and their features.
  • Evaluation of microbioreactors for high-throughput screening and process development.
  • Comparison of microbioreactors with traditional shake flask and microtiter plate methods.

Main Results:

  • Microbioreactors provide superior control and flexibility compared to traditional methods.
  • They offer high data density with online cultivation data, crucial for process optimization.
  • Microbioreactors enable results more comparable to large-scale fermentations.

Conclusions:

  • Microbioreactors represent a significant advancement for strain development in biotechnology.
  • Their controllability, automation potential, and data richness enhance industrial applicability.
  • Further research should focus on optimizing microbioreactor designs and applications for large-scale bioprocesses.